- King Dedede (antagonist) (Ed Paul/Ted Lewis)
- Escargoon (antagonist) (also, Ed Paul/Ted Lewis)
- Meta Knight (Eric Stuart)
- Tiff and Tuff (Kerry Williams as Tiff and Kayzie Rogers as Tuff)
- Sir Ebrum and Lady Like
- Fololo and Falala (Veronica Taylor)
- Sword Knight and Blade Knight
- The Cappys (featuring Mike Pollock as the mayor)
- Chef Kawasaki (Maddie Blaustein)
- Takori the Bird (cameo)
- The Waddle Dees (semi-antagonist)
- Nightmare Interprise Salesman (anatgonist) (Dan Green)
- Walky (antagonist)
- Contains reference to tea-cup ride.
- It was stated that Kirby's most dangerous attack is Mike Kirby.